abstract：:Binding of 3H-labeled 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor; PAF) to the intact, washed canine platelet has been defined and characterized as being specific and receptor-mediated. Under the conditions described, specific binding to 2 X 10(7) canine platelets reached saturation with...

journal_title：Thrombosis research

authors： Janero DR,Burghardt B,Burghardt C

更新日期：1988-06-15 00:00:00

abstract：:The effect of alpha-tocopherol on various endothelial cell functions was evaluated in vitro using cultures of human umbilical vein endothelial cells. Prostacyclin synthesis, plasminogen activator activity and von Willebrand factor activity were evaluated in control and alpha-tocopherol supplemented cultures. Alpha-toc...

journal_title：Thrombosis research

authors： Huang N,Lineberger B,Steiner M

更新日期：1988-05-15 00:00:00

abstract：:The Fab fragment of a polyclonal antibody against platelet factor XIII inhibited the collagen-induced platelet aggregation in a dose-dependent manner. This inhibitory effect was specific for collagen, and it had no effect on arachidonic acid-, ADP-, and serotonin-induced aggregations. This finding strengthens our noti...

journal_title：Thrombosis research

authors： Kasahara K,Takagi J,Sekiya F,Inada Y,Saito Y

更新日期：1988-04-15 00:00:00

abstract：:A quantitative assessment was made of the distribution of arachidonic acid (AA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA) among the individual sub-classes (diacyl, alkylacyl, alkenylacyl) of the various platelet phospholipids of human subjects consuming a fish oil concen...

journal_title：Thrombosis research

authors： Holub BJ,Celi B,Skeaff CM

更新日期：1988-04-01 00:00:00

abstract：:The abnormal Antithrombin-III (AT-III) Toyama has no ability to interact with heparin in the mast cells or heparin-like glycosaminoglycans on the surface of vascular endothelial cells, so that it has low heparin cofactor activity. In the present study, we have measured the binding capacity of 125I-labeled AT-III to cu...

journal_title：Thrombosis research

authors： Saito S,Takahashi K,Sakuragawa N

更新日期：1988-04-01 00:00:00

abstract：:We report here that the rate of fibrin polymerization is accelerated by decompression: the rate is essentially doubled by reducing the pressure by one-half (380 torr, 14.7 PSI or 1/2 ATM) and increased by 3.6 times with respect to the control at 25 torr (0.48 PSI or 1/30th ATM). We also report here that the rate of fi...

journal_title：Thrombosis research

authors： Murayama M

更新日期：1988-03-15 00:00:00

abstract：:The effect of a low molecular weight heparin (Fragmin) (20-80 U/kg) was studied in 10 healthy male volunteers after intravenous administration. Clotting abnormalities are extensive shortly after injection, lasting for 1-2 hours depending on heparin dosage. Dilute thrombin time, showing a close correlation to anti-F-Xa...

journal_title：Thrombosis research

authors： Andrassy K,Mörike K,Koderisch J,Weber E

更新日期：1988-03-15 00:00:00

abstract：:The protease inhibitor, aprotinin, has been examined for its ability to inhibit urokinase and tissue-type plasminogen activators at pH 7.4 in assays utilizing pyroGlu-Gly-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide as substrates, respectively. Aprotinin inhibited both two-chain low molecular weight urokinase...

journal_title：Thrombosis research

authors： Lottenberg R,Sjak-Shie N,Fazleabas AT,Roberts RM

更新日期：1988-03-15 00:00:00

abstract：:A series of 8 analogues of platelet-activating factor (PAF) in which both the acetyl group and the polar head are changed, were tested for biological activities on human and rabbit platelets in plasma. Two of these compounds, 1-O-hexadecyl-2-O-ethyl-rac-glycero-3-phosphoric acid-5'-trimethylammoniumpentyl ester and 1-...

journal_title：Thrombosis research

authors： Hofmann B,Ostermann G,Kertscher HP,Till U

更新日期：1988-02-15 00:00:00

abstract：:The proteinase, brinase (Mr approximately 35000), from Aspergillus oryzae, which has been used in therapeutic attempts as a thrombolytic agent in arterial thrombosis, binds to purified human alpha 2-macroglobulin (alpha 2M) with a stoichiometry of 1.7-1.9 mol of enzyme/mol inhibitor. This binding leads to quantitative...

journal_title：Thrombosis research

authors： Larsson LJ,Frisch EP,Törneke K,Lindblom T,Björk I

更新日期：1988-01-01 00:00:00

abstract：:The extrinsic fibrinolytic system was assessed among 124 consecutive survivors of acute myocardial infarction below 70 years of age. In samples drawn 3 months after discharge from hospital, the PAI-1 levels were higher and the tPA activities were lower than among elderly healthy controls. In contrast, the AMI survivor...

journal_title：Thrombosis research

authors： Nilsson TK,Johnson O

更新日期：1987-12-15 00:00:00

abstract：:Heat treatment is employed to diminish the transmission of hepatitis when blood products are administered. It is possible that such a procedure could reduce the biological activity of the proteins and induce changes in structure and aggregation state. We have therefore made in vitro and pharmacokinetic studies of heat...

journal_title：Thrombosis research

authors： Tengborn L,Frohm B,Nilsson LE,Johansson L,Andersson LO

更新日期：1987-12-15 00:00:00

abstract：:Time response curves of the anti-thrombotic effects, bleeding enhancing effects, effects on APTT, anti-Xa activities, anti-thrombin activities and thrombin generation inhibitory activities of the low molecular weight heparinoid Org 10172 and heparin have been compared in rats. The time courses of these effects were si...

journal_title：Thrombosis research

authors： Hobbelen PM,Vogel GM,Meuleman DG

更新日期：1987-12-01 00:00:00

abstract：:Previous studies indicated that human placental anticoagulant protein, a member of the lipocortin family, prolonged the clotting time of normal plasma when clotting was induced by brain thromboplastin or by kaolin in the presence of cephalin and calcium. Using a two-stage amidolytic assay to assess factor X activation...

journal_title：Thrombosis research

authors： Kondo S,Noguchi M,Funakoshi T,Fujikawa K,Kisiel W

更新日期：1987-11-15 00:00:00

abstract：:We have studied the synthesis and subcellular distribution of the glycosylated membrane protein tissue factor (TF) in human blood monocytes and the inducible monocyte like cell line, HL-60. Following induction with endotoxin or the phorbol ester, TPA, tissue factor specific activity was measured in intact cells, sonic...

journal_title：Thrombosis research

authors： Bona R,Lee E,Rickles F

更新日期：1987-11-15 00:00:00

abstract：:A tritium-labelled low molecular weight heparin fragment with an average molecular weight of 4000-6000 (Fragmin), was fractionated into its high and low affinity forms for antithrombin. The fractions obtained were injected into rabbits, and the plasma half-life (t1/2) volume of distribution (Vd), area under the curve ...

journal_title：Thrombosis research

authors： Palm M,Mattsson C

更新日期：1987-10-01 00:00:00

abstract：:Thrombus formation was electrically induced in the left common carotid artery of rats with preoccluded both vertebral and right carotid arteries. Following thrombus induction, animals became unresponsive and lost their righting reflex. The loss of righting reflex was observed when cortical tissular pO2 reached 55 +/- ...

journal_title：Thrombosis research

authors： Massad L,Plotkine M,Capdeville C,Boulu RG

更新日期：1987-10-01 00:00:00

abstract：:A new approach to the investigation of accumulation of acridine derivatives and release by platelets is proposed. At high concentrations of acridine orange (AO) inside dense bodies, a two component fluorescence spectrum with maxima at 530 and 640 nm has been recorded. Computer deconvolution of spectra provided additio...

journal_title：Thrombosis research

authors： Popov EG,Gabbasov ZA,Gavrilov IYu,Pozin EYa,Mejlumian AG

更新日期：1987-09-15 00:00:00

abstract：:The effect of the vasopressin analogue DDAVP on Factor VIII in plasma has been extensively studied in humans. To examine the effect of new and potentially better analogues a suitable animal model has to be established. In the present study trained Beagle dogs were injected with DDAVP and the time course of the Factor ...

journal_title：Thrombosis research

authors： Vilhardt H,Barth T,Falch J,Nilsson IM

更新日期：1987-09-01 00:00:00

abstract：:An anticoagulant activity was identified and isolated from the leaves of a West African plant, Aspilia africana by gel filtration on Sephadex G-100. The anticoagulant factor had an apparent molecular weight of approximately 60,000 d. Upon incubation with plasma, it prolonged the partial thromboplastin time, prothrombi...

journal_title：Thrombosis research

authors： Hanna MM,Niemetz J

更新日期：1987-08-15 00:00:00

abstract：:A 15 year-aged Japanese girl with a life long mild purpura was found as a variant type of thrombasthenia. Basic tests revealed prolonged bleeding time, border-line clot retraction, no coagulation defect, no giant platelets and mild thrombocytopenia (70,000-110,000/microliters). Neither ADP, epinephrine nor collagen ag...

journal_title：Thrombosis research

authors： Tanoue K,Hasegawa S,Yamaguchi A,Yamamoto N,Yamazaki H

更新日期：1987-08-01 00:00:00

abstract：:Anticoagulant properties of three sulfated compounds prepared from xylans isolated from corn cobs, larchwood and oatspelts were compared with heparin and sodium pentosan polysulfate (SP-54) by studying their effects on activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) using poo...

journal_title：Thrombosis research

authors： Campbell A,Nesheim ME,Doctor VM

更新日期：1987-08-01 00:00:00

abstract：:The potent inhibitor of platelet cAMP phosphodiesterase (PDE) HL 725 (9,10-Dimethoxy-2-mesitylimino-3-methyl-3, 4,6,7-tetrahydro-2H-pyrimido(6,1-A)-isoquinoline-4-one-hydrochloride), was examined for its effects on human and rat platelet aggregation. Strong inhibitory effects are seen on collagen-induced platelet aggr...

journal_title：Thrombosis research

authors： Agarwal KC,Buckley RS,Parks RE Jr

更新日期：1987-07-15 00:00:00

abstract：:The coumarin derivative AD6 is known to inhibit platelet aggregation and release and it possesses vasodilatory properties on coronary arteries of laboratory animals. Furthermore, the inhibition of the production of TxB2 from endogenous substrates after stimulation of human platelets with collagen has been demonstrated...

journal_title：Thrombosis research

authors： Porcellati S,Costantini V,Prosdocimi M,Pistolesi R,Porrovecchio P,Nenci GG,Goracci G

更新日期：1987-07-01 00:00:00

abstract：:This work demonstrates that after bolus intravenous injection standard heparin (SH) disappearance results from the combination of a saturable and a non saturable mechanism. Pharmacokinetics and pharmacodynamics of SH were studied by measuring the disappearance of increasing doses (5 - 500 anti-factor Xa U/kg) of 125I-...

journal_title：Thrombosis research

authors： Boneu B,Caranobe C,Gabaig AM,Dupouy D,Sie P,Buchanan MR,Hirsh J

更新日期：1987-06-15 00:00:00

abstract：:Nineteen patients, (2 adults, 17 children) with inherited bleeding disorders were infused with n-heptane-suspended-heated clotting factor concentrates. Twelve of the nineteen were previously untreated. Six patients were infused with Profilnine Heat-Treated and 13 with Profilate Heat-Treated. Five separate centers part...

journal_title：Thrombosis research

authors： Carnelli V,Gomperts ED,Friedman A,Aledort L,Hilgartner M,Dietrich S,Fedor EJ

更新日期：1987-06-15 00:00:00

abstract：:Many patients with diabetes mellitus show increased platelet aggregation and prostaglandin synthesis in response to physiological agents such as ADP and collagen when their platelets are tested in platelet-rich plasma or washed platelet suspensions. However, the relationship between increased platelet aggregation in v...

journal_title：Thrombosis research

authors： Ingerman-Wojenski CM,Smith M,Silver MJ

更新日期：1987-06-01 00:00:00

abstract：:Changes in cytoplasmic free calcium in response to epinephrine in combination with other agonists were studied in human platelets loaded with the fluorescent calcium indicator, quin 2. In the presence of 1mM external calcium, epinephrine augmented the increase in cytoplasmic free calcium in response to collagen or thr...

journal_title：Thrombosis research

authors： Ardlie NG,Bell LK,McGuiness JA

更新日期：1987-05-15 00:00:00

abstract：:It has previously been observed that aspirin diminishes the increase in blood fibrinolytic activity during arm venous occlusion. We studied the duration of this inhibitory effect on fibrinolytic response during 20 minutes arm venous occlusion and its effect on fibrinolytic response during acute physical activity (stan...

journal_title：Thrombosis research

authors： Keber I,Jereb M,Keber D

更新日期：1987-04-15 00:00:00

abstract：:The three polypeptide chains of fibrinogen, A alpha, B beta and gamma chain, are synthesized on separate polysomes. Fully formed fibrinogen is a six chain, disulfide-linked, dimeric molecule with a molecular weight of 340kDa. Previous pulse-chase studies with L-35 S methionine using the human hepatocellular carcinoma ...

journal_title：Thrombosis research

authors： Yu S,Kong V,Kudryk B,Redman C

更新日期：1987-04-15 00:00:00

abstract：:The effect of the combined oral administration of pentoxifylline (pof) and low dose acetylsalicylic acid (ASA) was evaluated with the help of the laser-induced thrombosis in rat mesenteric arterioles. Laser-induced thrombosis is inhibited in a dose-dependent way by both drugs. The administration of ASA, either simulta...

journal_title：Thrombosis research

authors： Seiffge D,Weithmann KU

更新日期：1987-04-15 00:00:00

abstract：:A synthetic pentasaccharide, representing the critical sequence required in heparin for binding to antithrombin III (AT III), produces strong anti-factor Xa activity in vitro in the presence of AT III and is devoid of any activity directed towards thrombin. This pentasaccharide provides a unique tool to study the ques...

journal_title：Thrombosis research

authors： Walenga JM,Petitou M,Lormeau JC,Samama M,Fareed J,Choay J

更新日期：1987-04-15 00:00:00

abstract：:Rabbit platelets pretreated with CV-3988 had reduced responsiveness to platelet activating factor(PAF) even after the platelets had been washed with a buffer. This was due to its tight binding to receptor sites for PAF, but not conformational changes of the receptor, because the platelets that were pretreated with CV-...

journal_title：Thrombosis research

authors： Tokumura A,Yoshida J,Okasaka N,Fukuzawa K,Tsukatani H

更新日期：1987-04-01 00:00:00

abstract：:It has been found in our laboratory that compression induces disaggregation and decompression induces aggregation of human platelets. The experimental design was based on the premise that oppositely charged amino acid residues of fibrinogen are juxtaposed in the specific adhesive site(s). This can be explained by the ...

journal_title：Thrombosis research

authors： Murayama M

更新日期：1987-03-15 00:00:00

abstract：:Separation of plasma, erythrocytes, and platelets was studied in T-shaped bifurcations prepared by a casting technique. The diameters of the side and main channel were 100 microns and 200 microns, respectively. Small blood samples (about 20 microliters) were collected from the side channel for determination of erythro...

journal_title：Thrombosis research

authors： Perkkiö J,Wurzinger LJ,Schmid-Schönbein H

更新日期：1987-03-01 00:00:00

abstract：:Two monoclonal antibodies against plasminogen and two against alpha-2-antiplasmin were characterized regarding their binding properties towards their respective native and modified antigens and towards the plasmin-alpha-2-antiplasmin complex. Using an ELISA system, the two monoclonal antibodies against plasminogen (MP...

journal_title：Thrombosis research

authors： Hattey E,Wojta J,Binder BR

更新日期：1987-03-01 00:00:00

abstract：:The procoagulant cellular activity (PCA) of leukemic cells was evaluated, before and after endotoxin stimulation, in 38 patients with acute leukemia at presentation subdivided according to the FAB classification. In the M4 and M5 subgroups the stimulated leukemic cells showed a significant increase in the production o...

journal_title：Thrombosis research

authors： Guarini A,Gugliotta L,Catani L,Chetti L,Macchi S,Tura S

更新日期：1987-03-01 00:00:00

abstract：:Forty women undergoing surgery under general anaesthesia for hyperplasia mammae were randomized to treatment with low dose heparin (5000 IU twice daily) or not. Preoperatively, and repeated on the 3. postoperative day, assays of euglobulin clot lysis time (ELT) after venous stasis, tissue plasminogen activator (t-PA) ...

journal_title：Thrombosis research

authors： Arnesen H,Engebretsen LF,Ugland OM,Seljeflot I,Kierulf P

更新日期：1987-03-01 00:00:00

abstract：:The influence of acute hypoxemia on blood fibrinolytic activity was investigated in 12 healthy males. Physiologically significant hypoxemia was produced by inspiration of 13% oxygen for 30 min. Six healthy males were exposed to hypoxemia at rest and 6 males to hypoxemia during exhaustive physical exercise on an ergocy...

journal_title：Thrombosis research

authors： Stegnar M,Peternel P,Chen JP

更新日期：1987-02-15 00:00:00

abstract：:Washed human platelets were incubated with commercial factor VIII concentrate, or with purified factor VIII coagulant moiety. Platelets were then washed again and lysed by sonication. VIII:Ag and vWf:Ag were measured in the platelet lysate prior to and after incubation of the lysate with phospholipase C (PL-C). Platel...

journal_title：Thrombosis research

authors： Muntean W,Leschnik B,Haas J

更新日期：1987-02-15 00:00:00